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pcdna3 1 sc35 cmyc  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 sc35 cmyc
    Pcdna3 1 Sc35 Cmyc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+sc35+mcherry/pRDAX-355+C-Tag+Venus173%2F155%5B2%5D+L10+%7BEsp3I(2x)%7D+mCherry+(Plasmid+%23129858)/pm36658339-507-15-16
    Average 91 stars, based on 1 article reviews
    pcdna3 1 sc35 cmyc - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: RNA sequences that induce fluorescence of small molecule fluorophores
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with the following primers: forward primer (SEQ ID NO: 62) 5′-ATA TAT GGA TCC AAT GGT GAG CAA GGG CGA GG-3′ reverse primer (SEQ ID NO: 63) 5′-TAT ATA TAA GCT TTC ACT TGT ACA GCT CGT CC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. Imaging Spinach-7SK and Spinach2-7SK: HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per milliliter.

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA.
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPCSpinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward primer 5′-ATATATGGATCCAATGGTGAGCAAGGGC GAGG-3′ and reverse primer 5′-TATATATAAGCTTTCACTTGT ACAGCTCGTCC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. ..

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat RNA
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward (5′-ATATATGGATCCAATGGTGAGCAAGGGCGAGG -3′) and reverse (5′-TATATATAAGCTTTCACTTGTACAGCTCGTCC -3′) primers and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per mL.

    Generated:

    Article Title: RNA sequences that induce fluorescence of small molecule fluorophores
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with the following primers: forward primer (SEQ ID NO: 62) 5′-ATA TAT GGA TCC AAT GGT GAG CAA GGG CGA GG-3′ reverse primer (SEQ ID NO: 63) 5′-TAT ATA TAA GCT TTC ACT TGT ACA GCT CGT CC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. Imaging Spinach-7SK and Spinach2-7SK: HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per milliliter.

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA.
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPCSpinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward primer 5′-ATATATGGATCCAATGGTGAGCAAGGGC GAGG-3′ and reverse primer 5′-TATATATAAGCTTTCACTTGT ACAGCTCGTCC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. ..

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat RNA
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward (5′-ATATATGGATCCAATGGTGAGCAAGGGCGAGG -3′) and reverse (5′-TATATATAAGCTTTCACTTGTACAGCTCGTCC -3′) primers and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per mL.

    Cloning:

    Article Title: RNA sequences that induce fluorescence of small molecule fluorophores
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with the following primers: forward primer (SEQ ID NO: 62) 5′-ATA TAT GGA TCC AAT GGT GAG CAA GGG CGA GG-3′ reverse primer (SEQ ID NO: 63) 5′-TAT ATA TAA GCT TTC ACT TGT ACA GCT CGT CC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. Imaging Spinach-7SK and Spinach2-7SK: HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per milliliter.

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA.
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPCSpinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward primer 5′-ATATATGGATCCAATGGTGAGCAAGGGC GAGG-3′ and reverse primer 5′-TATATATAAGCTTTCACTTGT ACAGCTCGTCC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. ..

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat RNA
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward (5′-ATATATGGATCCAATGGTGAGCAAGGGCGAGG -3′) and reverse (5′-TATATATAAGCTTTCACTTGTACAGCTCGTCC -3′) primers and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per mL.

    Plasmid Preparation:

    Article Title: RNA sequences that induce fluorescence of small molecule fluorophores
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with the following primers: forward primer (SEQ ID NO: 62) 5′-ATA TAT GGA TCC AAT GGT GAG CAA GGG CGA GG-3′ reverse primer (SEQ ID NO: 63) 5′-TAT ATA TAA GCT TTC ACT TGT ACA GCT CGT CC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. Imaging Spinach-7SK and Spinach2-7SK: HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per milliliter.

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat-containing RNA.
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPCSpinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward primer 5′-ATATATGGATCCAATGGTGAGCAAGGGC GAGG-3′ and reverse primer 5′-TATATATAAGCTTTCACTTGT ACAGCTCGTCC-3′, and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. ..

    Article Title: A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat RNA
    Article Snippet: .. The Spinach and Spinach2 PCR products were then ligated into pLPCXU6PT7SK-fixed at BamHI and BglII to generate pLPC-Spinach-7SK and pLPC-Spinach2-7SK, respectively. pSC35-mCherry was generated by amplifying mCherry with forward (5′-ATATATGGATCCAATGGTGAGCAAGGGCGAGG -3′) and reverse (5′-TATATATAAGCTTTCACTTGTACAGCTCGTCC -3′) primers and cloning via BamHI and HindIII digestion downstream of SC35 in pcDNA3.1-SC35-cMyc (Addgene plasmid 44721) to generate pcDNA3.1-SC35-mCherry. .. HeLa cells (ATCC-CRM-CCL-2) were cultured and passaged in DMEM medium supplemented with 50 units of penicillin and 50 μg of streptomycin per mL.



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